Get Full Essay Get access to this section to get all help you need with your essay and educational issues. Get Access Plant Tissue Culture Essay Sample Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition Any piece of tissue like a seed or stem tip that is placed in a nutrient-rich medium and allowed to grow is called a plant tissue culture.
In a standard tissue culture lab, there must be a few basic facilities like: A Media Room for preparation, sterilization and storage of culture media. Facilities for washing of lab-wares, explants, etc. Space for storage of lab-wares. Culture rooms or incubators where conditions of temperature, humidity and light etc.
Observation and Data Collection area. The formulation or the medium on which the explant is cultured is called culture medium. It is composed of various nutrients required for proper culturing.
Different types of plants and organs need different compositions of culture media. A number of media have been devised for specific tissues and organs. Some important of them are: Most preferred carbon source is Sucrose.
Others include lactose, maltose, galactose, raffinose, cellobiose, etc. Auxins-mainly for inducing cell division.
Cytokinins-mainly for modifying apical dominance and shoot differentiation. These are added to media to make them semisolid or solid. Agar, Gelatin, Alginate etc. Sometimes culture media are supplemented with some organic extracts also like coconut milk, orange juice, tomato juice, potato extract, etc.
Maintenance of aseptic conditions is the most critical and difficult aspect of in-vitro culturing experiments. Aseptic condition mean the conditions free from any type of microorganisms so as to prevent the loss of experiment by contamination.
For this, sterilization i. The most common contaminants in culture are fungi and bacteria. Measures to be taken for maintaining asepsis during tissue culture are: Sterilization of the culture vessels using detergents, autoclaves, etc. Sterilization of instruments like forceps, needles etc.
Sterilization of culture medium using filter sterilization or autoclaving methods.
The whole procedure of plant tissue culture is to be carried out essentially under aseptic conditions. So, the overall design of the laboratory must focus on the maintenance of aseptic conditions. While performing the tissue culture experiments there must present the first aid kits and fire extinguishers in the laboratory to avoid any mishap or accident.
In addition, proper attention should be given while handling the toxic chemicals and all the chemicals should be kept in correct labeled containers and bottles. General Technique of Plant Tissue Culture: General technique of plant cell, tissue and organ culture is almost the same with a little variation for different plant materials.
There are certain basic steps for the regeneration of a complete plant from an explant cultured on the nutrient medium Fig. These basic steps for in-vitro culturing of plants are: Suitable explant is selected and is then excised from the donor plant.
Explant is then sterilized using disinfectants.
A suitable culture medium is prepared with special attention towards the objectives of culture and type of explant to be cultured. Prepared culture medium is transferred into sterilized vessels and then sterilized in autoclave. Sterilized explant is inoculated transferred on the culture medium under aseptic conditions.As Lineberger (n.d) mentions, plant tissue culture can contributes to the agriculture industry in the future and give advantageous to the growers because the mass production of plant and crops can be produced in a short period of time using the tissue collected from single parent plant.
Plant Tissue Culture – Essay Sample Plants are made up of small component such as cells, organs or tissue that can be manipulated to grow complete plants.
Without molecular genetics and plant culture, it would be void to discuss genetic engineering as they are core to this. Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition.
Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Plant Tissue Culture Essay Sample. Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition Any piece of tissue like a seed or stem tip that is placed in a nutrient-rich medium and allowed to grow is called a plant tissue culture.
Essay # 3.
Techniques of Plant Tissue Culture: 1. Isolation of Plant Material: The plant from which tissue is to be removed is called stock plant. Removed plant part or tissue (2 to 4 mm 3 sterile segment) is known as explant.
Explants may be pieces of stem, leaf, root, flower, and fruit of seed. Plant tissue culture Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions I made a detail analysis of the literature and create the following essay which summons up the essence of the literature.